Analysis Note

ControlRBL cell lysate

Application

Western Blot Analysis: A 1:5,000 dilution from a representative lot detected FcεRI α subunit in 10 µg of human spleen tissue lysate.

Immunoprecipitation Analysis: A 1:50 dilution from a representative lot immunoprecipitated FcεRI α subunit from human spleen whole lysate.

Flow Cytometry Analysis: A 1:200 dilution from a representative lot detected FcεRI α subunit in RBL cells.

Research CategoryInflammation & Immunology

Detect FcεRI α subunit using this Anti-FcεRI α subunit Antibody validated for use in Western Blotting, Immunoprecipitation, ICC.

Research Sub CategoryInflammation & Autoimmune Mechanisms

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The allergic reaction is mediated through the IgE high affinity receptor (FcεRI). When a multivalent antigen is presented, a redistribution of the monomeric IgE FcεRI receptor complexes causes the degranulation of mast cells and basophils, resulting in the subsequent release of factors responsible for the allergic reaction. The FcεRI receptor is a tetrameric complex, consisting of an a-chain, b-chain and a dimeric g-chain. The a-chain is primarily involved with the binding of IgE, but the signal is transferred through the g subunit, which contains the immunoreceptor tyrosine activation motifs (ITAM) critical for initiating receptor mediated signal transduction through the Syk and Lyn tyrosine kinases. The FcεRI receptor is able to engage and disengage the IgE, providing a versatile model for studying the function of kinase coupled receptors.

Immunogen

Epitope: Extracellular domain

GST-tagged recombinant protein corresponding to the extracellular domain of human FcεRI α subunit.

Linkage

Replaces: 06-725

Physical form

Rabbit polyclonal serum containing 0.05% sodium azide.

Unpurified

Quality

Evaluated by Western Blot in RBL cell lysate.

Western Blot Analysis: A 1:5,000 dilution of this antibody detected FcεRI α subunit in 10 µg of RBL cell lysate.

Specificity

This antibody recognizes the extracellular domain of FcεRI α subunit.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~57 kDa observed